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glosensor camp reporter promega plasmid p22f  (Promega)

 
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    Structured Review

    Promega glosensor camp reporter promega plasmid p22f
    Glosensor Camp Reporter Promega Plasmid P22f, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/glosensor+camp+reporter+promega+plasmid+p22f/glosensor+camp+reporter+plasmid+p22f/pm38417010__ja3c09694_si_001-24-17-20
    Average 90 stars, based on 1 article reviews
    glosensor camp reporter promega plasmid p22f - by Bioz Stars, 2026-10
    90/100 stars

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    Derivative Assay:

    Article Title: Backbone Modification Provides a Long-Acting Inverse Agonist of Pathogenic, Constitutively Active PTH1R Variants
    Article Snippet: For cell assays, each peptide was dissolved in 10 mM aqueous acetic acid to the final concentration of 1 mM (the stock solution), Serine-26-O-glcosylated PTH(1-34) agonist peptide (Glc-PTH(1-34): SVSEIQLMHNLGKHLNSMERVEWLRS(glc)KLQDVHNF-NH2) was prepared as described previously.2 The radioligand 125I-LA-PTH (AVAEIQLnLHQRAKWIQDARRRAFLHKLIAEIHTAEY-NH2) 3 was prepared by chloramine T oxidation in the presence of 125I-Na (PerkinElmer, NEZ033H; 2,200 Curies/millimole) followed by reverse‐phase HPLC purification. .. SGS-72 cells are derived from the human osteoblastic cell line SaOS2 (ATCC-HTB85) by stable transfection with the GloSensor cAMP reporter (Promega, plasmid p22F) and express the endogenous human PTH1R. .. GP-2.3, GHR-10, and GTP-4 cells are derived from HEK-293 cells (ATCC-CRL-1573) by stable transfection with Glosensor and the wild-type human PTH1R (GP2.3 cells) or one of the constitutively active mutant receptors, hPTH1R-H223R (GHR-10 cells) or PTH1R-T410P (GTP-4 cells).4 SGS-72 cells were cultured in McCoy's 5A media supplemented with MEM Non-Essential Amino Acids Solution and 15% fetal bovine serum (FBS).

    Stable Transfection:

    Article Title: Backbone Modification Provides a Long-Acting Inverse Agonist of Pathogenic, Constitutively Active PTH1R Variants
    Article Snippet: For cell assays, each peptide was dissolved in 10 mM aqueous acetic acid to the final concentration of 1 mM (the stock solution), Serine-26-O-glcosylated PTH(1-34) agonist peptide (Glc-PTH(1-34): SVSEIQLMHNLGKHLNSMERVEWLRS(glc)KLQDVHNF-NH2) was prepared as described previously.2 The radioligand 125I-LA-PTH (AVAEIQLnLHQRAKWIQDARRRAFLHKLIAEIHTAEY-NH2) 3 was prepared by chloramine T oxidation in the presence of 125I-Na (PerkinElmer, NEZ033H; 2,200 Curies/millimole) followed by reverse‐phase HPLC purification. .. SGS-72 cells are derived from the human osteoblastic cell line SaOS2 (ATCC-HTB85) by stable transfection with the GloSensor cAMP reporter (Promega, plasmid p22F) and express the endogenous human PTH1R. .. GP-2.3, GHR-10, and GTP-4 cells are derived from HEK-293 cells (ATCC-CRL-1573) by stable transfection with Glosensor and the wild-type human PTH1R (GP2.3 cells) or one of the constitutively active mutant receptors, hPTH1R-H223R (GHR-10 cells) or PTH1R-T410P (GTP-4 cells).4 SGS-72 cells were cultured in McCoy's 5A media supplemented with MEM Non-Essential Amino Acids Solution and 15% fetal bovine serum (FBS).

    Plasmid Preparation:

    Article Title: Backbone Modification Provides a Long-Acting Inverse Agonist of Pathogenic, Constitutively Active PTH1R Variants
    Article Snippet: For cell assays, each peptide was dissolved in 10 mM aqueous acetic acid to the final concentration of 1 mM (the stock solution), Serine-26-O-glcosylated PTH(1-34) agonist peptide (Glc-PTH(1-34): SVSEIQLMHNLGKHLNSMERVEWLRS(glc)KLQDVHNF-NH2) was prepared as described previously.2 The radioligand 125I-LA-PTH (AVAEIQLnLHQRAKWIQDARRRAFLHKLIAEIHTAEY-NH2) 3 was prepared by chloramine T oxidation in the presence of 125I-Na (PerkinElmer, NEZ033H; 2,200 Curies/millimole) followed by reverse‐phase HPLC purification. .. SGS-72 cells are derived from the human osteoblastic cell line SaOS2 (ATCC-HTB85) by stable transfection with the GloSensor cAMP reporter (Promega, plasmid p22F) and express the endogenous human PTH1R. .. GP-2.3, GHR-10, and GTP-4 cells are derived from HEK-293 cells (ATCC-CRL-1573) by stable transfection with Glosensor and the wild-type human PTH1R (GP2.3 cells) or one of the constitutively active mutant receptors, hPTH1R-H223R (GHR-10 cells) or PTH1R-T410P (GTP-4 cells).4 SGS-72 cells were cultured in McCoy's 5A media supplemented with MEM Non-Essential Amino Acids Solution and 15% fetal bovine serum (FBS).



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    90
    Promega glosensor camp reporter promega plasmid p22f
    Glosensor Camp Reporter Promega Plasmid P22f, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/glosensor+camp+reporter+promega+plasmid+p22f/glosensor+camp+reporter+plasmid+p22f/pm38417010__ja3c09694_si_001-24-17-20
    Average 90 stars, based on 1 article reviews
    glosensor camp reporter promega plasmid p22f - by Bioz Stars, 2026-10
    90/100 stars
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